Clostridium perfringens

Description

The Clostridium perfringens real-time qPCR detection kit from Primerdesign provides detection of Clostridium perfringens, a spore-forming bacterium used as a key indicator of faecal contamination and of the effectiveness of water treatment, because its resistant spores persist where other indicators are removed. The TaqMan-based assay targets a conserved, species-specific region for sensitive detection directly from extracted DNA.

Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of pathogen load.

Key features of the Clostridium perfringens kit

• Real-time qPCR (TaqMan) detection of Clostridium perfringens
• Robust indicator of treatment efficacy and faecal contamination
• Sensitive detection of resistant spores
• Primer/probe mix and positive control template included
• Advanced format adds an internal extraction control and a quantification standard
• Compatible with standard real-time PCR instruments

It is ideally suited to drinking- and process-water testing and treatment validation. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.