Description
The Sugarcane mosaic virus (SCMV) real-time qPCR detection kit from Primerdesign provides specific detection of sugarcane mosaic virus, an aphid-transmitted potyvirus (RNA virus) that causes mosaic disease and significant yield loss in sugarcane, maize and other grasses. The assay uses one-step RT real-time PCR to detect a conserved region of the viral genome from extracted RNA, supporting clean-planting-material programmes and crop surveillance.
Each kit supplies a ready-to-use primer and probe mix with a positive control template and is run as a one-step RT-qPCR, providing a complete workflow from extracted RNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of viral load.
Key features of the Sugarcane mosaic virus (SCMV) kit
- One-step RT real-time qPCR detection of SCMV RNA
- Specific target with high analytical specificity
- Sensitive detection in plant tissue and planting material
- Primer/probe mix and positive control template included
- Advanced format adds an internal extraction control and a quantification standard
- Compatible with standard real-time PCR instruments
It is ideally suited to plant-pathology diagnostics, clean-planting-material certification and crop surveillance. Available in Standard (qualitative) and Advanced (quantitative) formats, with the complete protocol and result interpretation provided in the included IFU/handbook.
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