Acholeplasma laidlawii

Description

The Acholeplasma laidlawii real-time qPCR detection kit from Primerdesign enables rapid, sensitive and highly specific identification of Acholeplasma laidlawii, one of the most common mollicute (mycoplasma-like) contaminants of mammalian cell cultures, culture media, reagents and bioprocess water. Left undetected, A. laidlawii can alter cell behaviour and compromise both experimental and manufacturing results, making early molecular screening essential for reliable quality control. The TaqMan-based assay targets a conserved, species-specific genomic region and detects even low-level contamination within a single working day.

Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of pathogen load.

Key features of the Acholeplasma laidlawii kit

• Real-time qPCR (TaqMan) detection of Acholeplasma laidlawii
• Conserved, species-specific target with high analytical specificity
• Sensitive to low target copy numbers for early contamination control
• Primer/probe mix and positive control template included
• Advanced format adds an internal extraction control and a quantification standard
• Compatible with standard real-time PCR instruments

The kit supports cell-culture quality control, bioprocess and water monitoring, and routine bioscience contamination screening. It is available in Standard (qualitative) and Advanced (quantitative) formats, with full set-up, controls and result interpretation described in the included IFU/handbook.