Description
VeraSeq™ ULtra DNA Polymerase is an engineered, ultra-thermostable, uracil-literate polymerase designed to maximize the speed, accuracy, and length of DNA synthesis during sequencing template preparation. The result is a novel enzyme that can read through uracil, extend a kilobase of sequence in 15 seconds and has an accuracy 25 times higher than Taq DNA Polymerase.
Supplied in:
20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, stabilizer and 50% glycerol; pH 7.4 at 25°C.
Supplied with:
5X VeraSeq™ Buffer II (B7102L) and 5X VeraSeq™ GC Buffer (B7130L).
You can ask us about low-glycerol or hot-start formulations.
Performance
Polymerase properties
Extension rate: 15 seconds per kilobase at 72˚C
Proofreading (3′-5′ exo): Yes, strong
Nick-translation (5′-3′ exo): No
Fidelity: > 25X higher than Taq DNA Polymerase
Strand displacement: No
Thermostability: Highly thermostable
Able to extend an RNA primer: No
Extends from a nick: No
Generate blunt end products: Yes
Uracil read through: Yes
Storage temperature: –25°C to –15°C

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