Description
The Saccharomyces diastaticus real-time qPCR detection kit from Primerdesign provides specific detection of diastatic Saccharomyces (S. cerevisiae var. diastaticus), a wild yeast that spoils beer through super-attenuation, phenolic off-flavours and dangerous over-pressurisation or gushing. The TaqMan-based assay targets the diastatic STA1 marker for sensitive detection directly from extracted DNA, supporting early brewery intervention.
Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of the target sequence.
Key features of the Saccharomyces diastaticus kit
- Real-time qPCR (TaqMan) detection of diastatic Saccharomyces (STA1)
- Targets the diastatic marker linked to over-attenuation and gushing
- Sensitive detection of low-level wild-yeast contamination
- Primer/probe mix and positive control template included
- Advanced format adds an internal extraction control and a quantification standard
- Compatible with standard real-time PCR instruments
It is ideally suited to brewery quality control, yeast-management and packaging-safety monitoring. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.
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