Escherichia coli (Shiga toxin)

Description

The Escherichia coli (Shiga toxin) real-time qPCR detection kit from Primerdesign provides detection of Shiga-toxin-producing E. coli (STEC) by targeting the stx1 and stx2 toxin genes responsible for severe foodborne disease. Because Shiga-toxin genes define the hazard across multiple serotypes, this marker-based approach gives an inclusive, sensitive screen directly from extracted nucleic acid.

Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of pathogen load.

Key features of the Escherichia coli (Shiga toxin) kit

• Real-time qPCR (TaqMan) detection of Shiga-toxin (stx) genes
• Inclusive marker covering STEC across serotypes
• Sensitive detection supporting food-safety control
• Primer/probe mix and positive control template included
• Advanced format adds an internal extraction control and a quantification standard
• Compatible with standard real-time PCR instruments

It is ideally suited to food-safety testing, outbreak investigation and quality control. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.