Clostridium perfringens (alpha-toxin producing)

Description

The Clostridium perfringens (alpha-toxin producing) real-time qPCR detection kit from Primerdesign provides specific detection of alpha-toxin-producing Clostridium perfringens, a spore-forming bacterium and frequent cause of foodborne illness associated with meat and cooked products held at improper temperatures. The TaqMan-based assay targets the alpha-toxin (cpa/plc) gene for sensitive, specific detection directly from extracted nucleic acid.

Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of pathogen load.

Key features of the Clostridium perfringens (alpha-toxin producing) kit

• Real-time qPCR (TaqMan) detection of alpha-toxin-producing C. perfringens
• Targets the alpha-toxin gene with high specificity
• Sensitive detection supporting food-safety control
• Primer/probe mix and positive control template included
• Advanced format adds an internal extraction control and a quantification standard
• Compatible with standard real-time PCR instruments

It is ideally suited to food-safety testing, processing-environment monitoring and quality control. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.