Description
The Clostridium difficile (toxin B) real-time qPCR detection kit from Primerdesign provides specific detection of toxin B-producing Clostridioides difficile, complementing toxin A testing for this spore-forming pathogen in water and environmental samples. The TaqMan-based assay targets the toxin B (tcdB) gene for sensitive, specific detection directly from extracted DNA.
Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of pathogen load.
Key features of the Clostridium difficile (toxin B) kit
- Real-time qPCR (TaqMan) detection of C. difficile toxin B (tcdB)
- Toxin-gene-specific target with high specificity
- Sensitive detection of a persistent, spore-forming organism
- Primer/probe mix and positive control template included
- Advanced format adds an internal extraction control and a quantification standard
- Compatible with standard real-time PCR instruments
It is ideally suited to water- and environmental-safety testing and surveillance. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.


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