Pichia kudriavzevii

Description

The Pichia kudriavzevii real-time qPCR detection kit from Primerdesign provides specific detection of Pichia kudriavzevii (formerly Issatchenkia orientalis), a spoilage yeast that forms surface films and produces off-flavours and volatile acidity in wine and other beverages. The TaqMan-based assay targets a species-specific genomic region for sensitive detection directly from extracted DNA, enabling early winery intervention.

Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of the target sequence.

Key features of the Pichia kudriavzevii kit

• Real-time qPCR (TaqMan) detection of Pichia kudriavzevii
• Species-specific target with high analytical specificity
• Sensitive detection of low-level spoilage-yeast contamination
• Primer/probe mix and positive control template included
• Advanced format adds an internal extraction control and a quantification standard
• Compatible with standard real-time PCR instruments

It is ideally suited to winery quality control, cellar hygiene and finished-wine monitoring. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.