Dekkera bruxellensis

Description

The Dekkera bruxellensis real-time qPCR detection kit from Primerdesign provides specific detection of Dekkera bruxellensis (Brettanomyces), the most significant spoilage yeast in winemaking, responsible for the ‘Brett’ faults of volatile phenols such as 4-ethylphenol. The TaqMan-based assay targets a species-specific genomic region for sensitive detection directly from extracted DNA, allowing intervention before sensory thresholds are exceeded.

Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of the target sequence.

Key features of the Dekkera bruxellensis kit

• Real-time qPCR (TaqMan) detection of Dekkera bruxellensis
• Species-specific target with high analytical specificity
• Sensitive detection before ‘Brett’ faults become perceptible
• Primer/probe mix and positive control template included
• Advanced format adds an internal extraction control and a quantification standard
• Compatible with standard real-time PCR instruments

It is ideally suited to winery quality control, barrel-management and finished-wine monitoring. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.