Brucella species

Description

The Brucella species real-time qPCR detection kit from Primerdesign provides specific detection of Brucella, the bacterial cause of brucellosis, a serious zoonosis transmitted through unpasteurised dairy and infected animal products. The TaqMan-based assay targets a conserved region of the genus for sensitive detection directly from extracted nucleic acid, supporting food-safety and veterinary surveillance for this hazardous, slow-growing pathogen.

Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of pathogen load.

Key features of the Brucella species kit

• Real-time qPCR (TaqMan) genus-level detection of Brucella
• Broad coverage of zoonotic Brucella species
• Sensitive detection avoiding hazardous culture of live organisms
• Primer/probe mix and positive control template included
• Advanced format adds an internal extraction control and a quantification standard
• Compatible with standard real-time PCR instruments

It is ideally suited to dairy and food-safety testing, veterinary diagnostics and surveillance. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.