Description
The Shiga toxin (stx2A) producing Escherichia coli real-time qPCR detection kit from Primerdesign provides specific detection of Shiga-toxin-producing E. coli (STEC) carrying the stx2A gene, a subtype strongly associated with severe disease including haemolytic uraemic syndrome. The TaqMan-based assay targets the stx2A gene for sensitive, specific detection directly from extracted nucleic acid.
Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of pathogen load.
Key features of the Shiga toxin (stx2A) producing Escherichia coli kit
- Real-time qPCR (TaqMan) detection of the stx2A toxin gene
- Specifically targets stx2A-carrying STEC
- Sensitive detection supporting food-safety and outbreak work
- Primer/probe mix and positive control template included
- Advanced format adds an internal extraction control and a quantification standard
- Compatible with standard real-time PCR instruments
It is ideally suited to food-safety testing, outbreak investigation and quality control. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.
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