Description
The Enterohaemorrhagic Escherichia coli (EHEC) real-time qPCR detection kit from Primerdesign provides specific detection of EHEC, the Shiga-toxin-producing E. coli group responsible for severe foodborne illness, including haemorrhagic colitis and haemolytic uraemic syndrome. The TaqMan-based assay targets the virulence markers characteristic of EHEC for sensitive, specific detection directly from extracted nucleic acid.
Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of pathogen load.
Key features of the Enterohaemorrhagic Escherichia coli (EHEC) kit
- Real-time qPCR (TaqMan) detection of EHEC virulence markers
- Specific to the enterohaemorrhagic pathotype
- Sensitive detection supporting food-safety control
- Primer/probe mix and positive control template included
- Advanced format adds an internal extraction control and a quantification standard
- Compatible with standard real-time PCR instruments
It is ideally suited to food-safety testing, outbreak investigation and quality control. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.
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