Description
The Escherichia coli (Tellurite Resistant) real-time qPCR detection kit from Primerdesign detects tellurite-resistance (ter) determinants in Escherichia coli. Tellurite resistance is carried by several pathogenic E. coli, including enterohaemorrhagic serotypes such as O157:H7, and potassium tellurite is widely used in selective culture media; this molecular assay targets the ter determinants as a rapid alternative to culture-based selection. The TaqMan-based assay gives sensitive, specific detection directly from extracted nucleic acid.
Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of pathogen load.
Key features of the Escherichia coli (Tellurite Resistant) kit
- Real-time qPCR (TaqMan) detection of tellurite-resistance (ter) determinants
- Marker associated with pathogenic, tellurite-resistant E. coli
- Sensitive detection as a rapid alternative to tellurite selective culture
- Primer/probe mix and positive control template included
- Advanced format adds an internal extraction control and a quantification standard
- Compatible with standard real-time PCR instruments
It is ideally suited to food- and water-safety testing, STEC screening support and quality control. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.
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