Description
The Enteropathogenic Escherichia coli (EPEC) real-time qPCR detection kit from Primerdesign provides specific detection of EPEC, an E. coli pathotype and major cause of infantile diarrhoea that can be transmitted through contaminated food and water. The TaqMan-based assay targets the attaching-and-effacing virulence markers characteristic of EPEC for sensitive, specific detection directly from extracted nucleic acid.
Each kit supplies a ready-to-use primer and probe mix together with a positive control template, providing a complete workflow from extracted DNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of pathogen load.
Key features of the Enteropathogenic Escherichia coli (EPEC) kit
- Real-time qPCR (TaqMan) detection of EPEC virulence markers
- Specific to the enteropathogenic pathotype
- Sensitive detection supporting food-safety control
- Primer/probe mix and positive control template included
- Advanced format adds an internal extraction control and a quantification standard
- Compatible with standard real-time PCR instruments
It is ideally suited to food- and water-safety testing, outbreak investigation and quality control. Available in Standard (qualitative) and Advanced (quantitative) formats, with detailed set-up and result interpretation described in the included IFU/handbook.
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