Piscine myocarditis virus (PMCV)

Description

The Piscine myocarditis virus (PMCV) real-time qPCR detection kit from Primerdesign provides specific detection of PMCV, the totivirus-like RNA virus associated with cardiomyopathy syndrome (CMS), a serious heart disease of farmed Atlantic salmon that typically strikes large fish near harvest. The assay uses one-step RT real-time PCR to detect a conserved region of the viral genome from extracted RNA.

Each kit supplies a ready-to-use primer and probe mix with a positive control template and is run as a one-step RT-qPCR, providing a complete workflow from extracted RNA on any standard real-time PCR platform. The Advanced format additionally includes an internal extraction control to flag inhibition and a copy-number standard curve for absolute quantification of viral load.

Key features of the Piscine myocarditis virus kit

• One-step RT real-time qPCR detection of PMCV RNA
• Specific target with high analytical specificity
• Sensitive detection supporting CMS diagnosis and monitoring
• Primer/probe mix and positive control template included
• Advanced format adds an internal extraction control and a quantification standard
• Compatible with standard real-time PCR instruments

It is ideally suited to salmon aquaculture diagnostics, heart-health investigation and biosecurity surveillance. Available in Standard (qualitative) and Advanced (quantitative) formats, with the complete protocol and result interpretation provided in the included IFU/handbook.